⑴ produce a lactate fermentation process, as close to neutral pH value of the fermentation. Need a certain lactate into Calcium lactate, lactate by direct addition of sulfuric acid to the solution, and can be made of lactic acid, a byproduct of the crystalline dihydrate of calcium sulfate. Was removed by filtration methods can, of course, can be used as calcium sulphate dihydrate gypsum ground perfusion, such as dry wall, cement raw materials and agricultural fields. Gypsum is generated in the production process of the low value of the salt, but this method is cost effective, because calcium hydroxide and sulfuric acid, low cost, and gypsum can be used for other industrial purposes. Others will alkalization and acidification processes linked to the two methods have been tried, for example, buffered lactic acid, adjusting pH, acidified with sulfuric acid to obtain a by-product ammonium sulfate, ammonium sulfate can be used as fertilizer. Because ammonium hydroxide, calcium than high prices, while high-value by-product ammonium sulfate just to make up this gap, and with respect to calcium sulfate soluble in water, which facilitates separation.
⑵ cell removal
Cell removal method of choice depends on the microorganism used in the production. Rhizopus oryzae length 210-2500μm, diameter 5-18μm, because of the smaller cells can be removed through flocculation. Chitosan in the fermentation solution by adding a flocculant, adjusted to ph 6.8, heat, stirring raising flocculation, coagulation was allowed to stand for 1.5h after the end of the supernatant in the centrifuge tube, and centrifuged at 4000r/min centrifuge 20min, the solid precipitate was isolated.
⑶ residual sugar, medium and fermentation residue separation of byproducts.
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